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Map of SIV vector constructs and gag-pol expression plasmids. Viral open reading frames and intact regulatory elements are marked by white boxes. GFP, coding region for the green fluorescent protein; CMV, human cytomegalovirus immediate-early promoter. The arrows mark the primer binding sites of U5 SIV LTRs 0 and U5 SIV LTRs 1; KpnI and DpnII restriction sites relevant for the LM-PCR are also shown (not all DpnII sites are shown).
