TABLE 3.
Transcriptional activities of targeted genesa
| Vector | % Integration within active RefSeq genes | Median frequency of tags of hit RefSeq genesc | 25th percentile | 75th percentile |
|---|---|---|---|---|
| SIVwt | 25.3 | 6.5 | 4 | 14 |
| SIVΔprom | 28.8 | 9 | 4 | 23 |
| SIVΔfrxn | 33.3 | 9 | 4 | 23 |
| SIV total | 28.8 | 9 | 4 | 19 |
| Random | 9.0b | 9 | 4 | 23 |
As an estimate for the transcriptional activity of RefSeq genes in 293 target cells, the representation of the RefSeq genes in a SAGE library was retrieved from the SAGE Genie website (http://cgap.nci.nih.gov/SAGE, library SAGE_Kidney_embryonic_CL_293-control). RefSeq genes represented in this library at least once were considered transcriptionally active. The median frequency of the tags of transcriptionally active hit genes per 200,000 tags in this SAGE library was compared to those of 200 randomly picked RefSeq genes. There is no significant difference between the different SIV vector particles.
P was <0.001 compared to SIV total (χ2 test).
There is no significant difference between the three constructs and the random data (Kruskal-Wallis test).