FIG. 1.

HPV11 L2 forms a complex with the Kap α₂β₁ heterodimer and interacts with the Kap β₁, Kap β₂, and Kap β₃ import receptors. Purified HPV11 L2 was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane. (A) One L2 blot was detected with anti-His antibody (lane 1). Other L2 blots were incubated with increasing concentrations of Kap α₂ in the absence (lanes 2 to 4) or in the presence (lanes 5 to 7 and 9 to 11) of 5 μg/ml GST-Kap β₁. For lane 12, the L2 blot was incubated with Kap α₂ and GST-Kap β₁ plus RanGTP. Separate L2 blots were incubated with GST-Kap β₁ alone (lane 8) or GST (lane 13). Bound Kap α₂ was detected with an anti-Kap α₂ antibody (Ab) (lanes 2 to 7), and bound GST-Kap β₁ and GST were detected with an anti-GST Ab (lanes 8 to 13). (B) L2 blots were incubated with either anti-His Ab (lane 1) or with 5 μg/ml GST and then anti-GST Ab (lane 2). (C) L2 blots were incubated with increasing concentrations of GST-Kap β₁ (lanes 1 to 4) or with GST-Kap β₁ plus RanGTP (lane 5), and the bound GST-Kap β₁ was detected with an anti-GST Ab. For panels D and E, similar incubations were done with GST-Kap β₂ and GST-Kap β₃, respectively.