FIG. 2.

Both the nNLS and the cNLS of HPV11 L2 can mediate nuclear import via a classical pathway. Digitonin-permeabilized HeLa cells were incubated with 0.25 μg of either GST-nNLS_11L2 (panels A to C), GST-cNLS_11L2 (panels D to F), GST-NLS_16L1 (panels G to I), or GST (panels J to L) in the presence of either transport buffer only (panels A, D, G, and J), HeLa cytosol (panels B, E, H, and K), or Kap α₂β₁ heterodimers (1 μg each) plus RanGDP (3 μg) (panels C, F, I, and L). Note the nuclear import in panels B, C, E, F, H, and I.