FIG. 3.

The nNLS and cNLS of HPV11 L2 can independently mediate nuclear localization of L2 in vivo. HeLa cells were transfected with either EGFP-L2 (A, panels A and B), EGFP (A, panels C and D), EGFP-L2ΔN (B, panels A and B), EGFP-L2ΔC (B, panels C and D), or EGFP-L2ΔNΔC (B, panels E and F) plasmids, using FUGENE 6, and were examined by fluorescence microscopy at 24 h posttransfection. The fluorescence of the EGFP is shown in the left column and DAPI (4′,6′-diamidino-2-phenylindole) staining of the nuclei is shown in the right column.