FIG. 3.

(A) ICP0 has no effect on unmodified Sp100 during wt HSV-1 infection of cells lacking PML. siC and siPML2 cells were infected with wt HSV-1 (MOI of 2), and then samples were harvested at 4 and 6 h after infection for analysis by Western blotting for Sp100, actin, ICP0, and UL42 as indicated. (B) The loss of the higher-molecular-weight isoforms of Sp100 during wt HSV-1 infection does not occur in ICP0-null mutant dl1403-infected cells. siC and siPML2 cells were infected with dl1403 at an MOI of 2, and samples were taken at the indicated times after infection for analysis by Western blotting for Sp100, actin, ICP4, and UL42. hpi, hours postinfection. Numbers at left are molecular masses in kilodaltons. m, mock infected.