FIG. 8.

Enhancement of dl1403 gene expression and plaque formation in cells expressing a different anti-PML shRNA. HF cells were transduced with lentiviruses expressing control GFP or anti-PML shRNA 3869. m, mock infected. (A) Cells were infected with dl1403 at an MOI of 2, and samples were harvested at the indicated time points. Expression of ICP4, UL42, and actin was detected by Western blotting. (B) PML expression in HF-3869 and HF-GFP cells compared to actin. (C) Relative plaque-forming efficiency of dl1403 in HF-3869 cells, relative to that in HF-GFP cells. The increase in plaque formation in HF-3869 cells averaged 3.0-fold from four replicate experiments, with a range of 2.0 to 3.6.