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Flow cytometric analysis of D2F2/E2 cells transfected with an expression vector expressing either the initial (A) or the adapted (B) chimeric Sindbis gp. Cells were stained with the mouse polyclonal anti-Sindbis antibody, VR1248, followed by a fluorescein isothiocyanate-conjugated goat anti-mouse IgG antibody. Control plots from cells stained only with the secondary antibody are outlined on the left of each figure. Results are shown for one representative experiment of 6 replicates.
