FIG. 6.

Correlation of viral infectivity with frequency of mutations and amounts of reverse transcripts. Correlation plots of viral infectivity (as the percent infectivity relative to virus produced in the absence of any APOBEC protein) versus the average number of mutations per 100 bp of sequenced viral cDNA (A), the percentage of clones sequenced that had at least one mutation (B), and the relative amount of early reverse transcripts compared to the amount of cDNA in the absence of any APOBEC protein (C), as measured by quantitative PCR, were prepared. Each point represents an individual viral preparation, and different experiments are identified by different symbols: panels A and B show data from experiments 1 (⧫) and 4 (•), and panel C shows the results of experiments 2 (▪), 3 (▴), and 4. The Pearson r value gives a measure of the correlation between each pair of variables. P is the probability that this correlation occurs by chance. Statistics were calculated by using GraphPad Prism version 4.0c.