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. 2006 Sep;80(17):8459–8468. doi: 10.1128/JVI.00545-06

FIG. 1.

FIG. 1.

The estradiol-inducible ToMV infection system. (A) Schematic representation of the estradiol-inducible infection system for a ToMV derivative that carries the ER-targeted GFP (erGFP) gene. A modified ToMV cDNA was placed between an estradiol-inducible promoter and a ribozyme sequence (Rz) followed by a polyadenylation signal (Ter). Upon estradiol induction, a modified ToMV RNA is produced via transcription by cellular RNA polymerase II followed by ribozyme cleavage. This modified ToMV RNA is translated to produce the 130K and 180K replication proteins and subjected to replication. A subgenomic mRNA encoding erGFP is synthesized during replication. (B to E) Estradiol-induced ToMV infection in transgenic BY-2 cells. Nomarski and GFP fluorescence images are superimposed. (B) Uninduced cells; (C) induced cells (2 days after induction); (D) protoplasts prepared from induced cells; (E) evacuolated protoplasts prepared from induced cells. Bars, 25 μm.