FIG. 1.
Genetic analysis of WT, UL49−, and UL49R viral DNAs. (A) Schematic representation of the BamHI F fragment in WT and UL49R (upper) and UL49− (lower) viral genomes. Replacement of the UL49 gene with a FRT-BamHI cassette in the UL49− viral genome yielded 6,512-bp and 724-bp BamHI fragments in place of the 8,054-bp BamHI F-fragment present in the WT and UL49R viral genomes. (B) Negative scanned image of an agarose gel containing BamHI-digested WT, UL49−, and UL49R viral DNAs visualized with ethidium bromide staining. (C) Scanned autoradiograph of Southern DNA hybridization of the same gel in which [α-32P]dCTP-labeled HSV-1 BamHI F-fragments were used as probes.