FIG. 4.

shRNA directed against Derlin-2 but not Derlin-1 impairs Py infectivity. (A) Immunoblot analysis, using the antibodies shown, of equivalent microgram quantities of lysates from C6 cells expressing the indicated shRNA construct. Derlin-1 and Derlin-2 shRNA constructs specifically reduced the corresponding protein levels. p97 served as an internal loading control. (B) The same C6 cells as those in panel A were examined for Py infectivity by staining for large T antigen as described in the legend of Fig. 1. The data shown reflect the means and standard deviations of Py infectivity determined from multiple independent experiments.