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. 2006 Sep;80(17):8329–8344. doi: 10.1128/JVI.00540-06

FIG. 1.

FIG. 1.

MP confers increased resistance to microtubule-destabilizing drugs in planta. Transgenic N. benthamiana plants expressing tua-GFP were infected with TMV-MP-mRFP1, and cells undergoing different stages of infection were analyzed by fluorescence microscopy before and after infiltration with 20 μM oryzalin. (A and B) In untreated cells undergoing later stages of infection, MP-mRFP1 (B) clearly associates with tua-GFP-labeled microtubules (A). (C) tua-GFP-labeled microtubules are readily apparent in noninfected, noninfiltrated control cells. (D) Mock infiltration with water does not disrupt tua-GFP-labeled microtubules in noninfected cells. (E and F) In cells at the front of the radially expanding TMV-MP-mRFP1 infection site, infiltration with oryzalin for 30 min leads to disruption of tua-GFP-labeled microtubules (E) and no association of MP-mRFP1 with microtubules can be observed (F). (G and H) Following treatment for 30 min with oryzalin, MP-mRFP1 (H) remains associated with stable tua-GFP-labeled microtubules (G) in cells behind the front of infection. Bar, 15 μm.