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. 2006 Sep;80(17):8329–8344. doi: 10.1128/JVI.00540-06

FIG. 3.

FIG. 3.

FRAP of microtubule-associated MP-GFP and GFP-MAP65-5. (A) FRAP of microtubule-associated MP-GFP in BY-2 protoplasts infected with TMV-MP-GFP occurs uniformly within the bleached portion of the microtubules (box). MP-GFP does not visibly move from unbleached areas along the length of microtubules. (B) Microtubule-associated fluorescence recovers similarly in BY-2 cells ectopically expressing MP-GFP. (C) Characteristic of a structural MAP, GFP-MAP65-5 fluorescence recovers in a uniform manner within the bleached portion of microtubules. Bars, 5 μm.