FIG. 4.

Dot blot hybridization of genomic DNA isolated from ASFV-infected EST-expressing clones with ASFV DNA, as a measure of ASFV replication. Genomic DNA was isolated from BAT3 sense and antisense clones. One hundred nanograms of total DNA was diluted serially at 1:2, and 100-μl samples of the increasing dilutions were transferred to wells from the left to right orientation (columns 1 to 6). One hundred nanograms of DNA was spotted in wells marked 1, half of that amount was spotted in wells numbered 2, etc. Malawai (Mal) DNA is the ASFV viral DNA that served as a positive control; genomic DNA from a parental HeLa cell line was used as a negative control.