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. 2006 Sep;80(17):8705–8717. doi: 10.1128/JVI.00475-06

FIG. 5.

FIG. 5.

cDNA sequence corresponding to BAT3 EST DNA and to the ORF of the fused BAT3 peptide. (A) The sequence of the BAT3 EST in the sense clone is bracketed by a pair of universal primers: the AEK reverse primer in the lined box is located at the 5′ side of the BAT3 sense sequence, and the AEK forward primer in the dotted box is positioned at the 3′ side of the BAT3 EST. The translational initiation codon of the BAT3 dominant peptide is underlined. (B) Putative ORF of the BAT3 peptide fused to a vector-encoded peptide corresponding to a segment of a woodchuck hepatitis virus DNA polymerase. The dotted box encompassing 71 amino acids is derived from the BAT3S EST, and the dashed box is the sequence of woodchuck hepatitis virus DNA polymerase encoded by the pLenti vector. The box formed by solid lines shows the NheI site where the EST was introduced into the vector, and the underlined sequence is the universal primer AEK forward, which is located on the 3′ side of the BAT3 EST sequence. The numbering at the left and right sides of the figure corresponds to amino acid and nucleotide sequence numbers of the ORF. The putative peptide contains 298 amino acids.