FIG. 7.

Reintroduction of wt p53 but not mutant p53 into p53^−/− cells restores UL44 focus formation and pp65 relocalization toward wt. (A) UL44 focus appearance and development were assayed via IF as described in the text and in the legend to Fig. 4. Percentages for HFF, THF, and p53^−/− cells are the same as presented in Fig. 4. (B) pp65 movement into the cytoplasm was assessed via IF as described in the text and in the legend to Fig. 5. Percentages for HFF, THF, and p53^−/− cells are the same as presented in Fig. 5. Assays were performed at least twice for each cell type. Error bars represent 1 standard deviation. pCDNA4 is a representative clone that contains only the pPUR-pCDNA3 backbone construct. R273Hpool and H179Qpool are pools of cells with mutant constructs reintroduced into the p53^−/− background. pCDNApool is a pool of cells with the backbone construct reintroduced into the p53^−/− cells.