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Resistance selection in HIV-1(IIIB)-infected (A and B) or HIV-1 (NL-4.3)-infected (C) CEM cell cultures in the presence of escalating concentrations of CV-N. Every 4 to 5 days, the cell cultures were subcultivated by adding 100 or 200 μl of the virus-infected cell suspension to 900 or 800 μl fresh culture medium, respectively.
