FIG. 5.
V5-tagged, mutated versions of the B5 protein. (A) Schematic representation of V5-tagged B5 versions used in this study. SP, signal peptide; V5, V5 epitope; R, four short consensus repeats (SCR I to IV); S, stalk; T, transmembrane domain; C, cytoplasmic tail; aa, amino acids. Versions were termed, as indicated on the left, according to the domains included in the construct. TG indicates the transmembrane domain of VSV G protein. R* indicates an R region including a P189S mutation located in SCR IV. (B) Predicted topology of mutated B5 versions in the outer membrane of the EEV particle. Domains above the lipid bilayer are in the lumen of the wrapping membranes or the extracellular space, whereas domains below the membrane are cytosolic. The N and C termini are indicated.