Recognition of gp140s by serum antibodies. Sera collected 4 weeks following the recombinant gp140 immunization step from the immunized animals, from the SHIVSF162P4-infected macaque, C640, at day 643 postinfection and HIVIG were added to ELISA wells coated with the indicated gp140s, and the binding of serum IgG was determined. Sera from each immunized group were pooled. Preimmunization or preinfection sera were used as a control for nonspecific binding, and the means with standard deviation of results from three separate experiments are shown.