FIG. 6.

Stimulation by Dhvar2 is dependent on HIV-1 Env. (A) SupT1 T cells were infected with a lentiviral vector in the presence of Dhvar2. The vector with the GFP gene was pseudotyped with either HIV-1 Env or VSV-G. After 3 days, the percentage of GFP-positive cells was determined by FACS. Representative FACS plots are shown, with the percentage of GFP-positive cells indicated. (B) SupT1 or LuSIV cells were infected with the lentiviral vector bearing HIV-1 Env, which was mock treated or incubated with Dh-5, Dhvar2, or Dhvar4. Three days later, the percentage of GFP-positive cells was determined.