FIG. 3.

CLEC-2 binds HIV-1, and bound virus is infectious for adjacent target cells. (A) B-THP cell lines were incubated with the indicated inhibitors and pulsed with p24-normalized HIV-1 NL4-3 Luc. Unbound virus was removed, cells were lysed, and the amount of p24 antigen in lysates was determined. The amount of virus recovered is expressed as a percentage of the input virus. A representative experiment is shown. Similar results were obtained in three independent experiments. (B) The experiment was conducted as described for panel A. However, after removal of unbound virus, platelets were cocultivated with CEMx174 R5 target cells (+) and luciferase activities in cell lysates determined 3 days after cocultivation. A representative experiment is shown. Results were confirmed in three independent experiments. Error bars represent standard deviations. P values were determined using a two-sided dependent-sample t test. IgG, immunoglobulin G; c.p.s., counts per second.