FIG. 7.

Megakaryocytes (arrows in panels A and B) and platelets (C and D) express DC-SIGN. (A to D) Sections of human bone marrow were immunostained (using the methodology described in the legend for Fig. 2) with anti-DC-SIGN (B and D) or anti-CD61 (A and C). DC-SIGN stains megakaryocytes (B, arrows) and a subpopulation of platelets (D, arrows). (E) Platelets were stained with the indicated anti-DC-SIGN MAb, and staining was analyzed by flow cytometry. Similar results were obtained in three independent experiments with different platelet preparations. IgG, immunoglobulin G; FL4-H, fluorescence in channel 4-height.