FIG. 2.

Detection of Tat and agnoprotein in cytoplasmic and nuclear fractions. Nuclear and cytoplasmic proteins were prepared from equal numbers of U-87MG cells cotransfected with YFP-agnoprotein and CFP-Tat-expressing plasmids as indicated above each lane. Protein extracts prepared from an equal number of cells were analyzed by a series of Western blots using antiagnoprotein antibody (24), anti-HIV-1 Tat (R705 from the AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH), antitubulin antibody, and anti-lamin A antibody.