FIG. 1.

G→A substitutions in HIV-1 proviral DNA with inordinate G→A preference and G→A burden occur predominantly in the dinucleotide sequence contexts targeted by APOBEC3G (GG) and, to a lesser extent, in those targeted by APOBEC3F (GA). (A) G→A preference (#G→A substitutions/all mutations) was highly significantly correlated to G→A burden (#G→ substitutions/#consensus G) (r = 0.853; P < 0.001). (B and C) The general G→A hypermutation scores were significantly correlated with the 3G-specific G→A substitution scores (r = 0.318; P < 0.001) but not with the 3F-specific G→A substitution scores (r = 0.065; P = 0.465). Red shaded circles, HM-3G sequences determined by mixed population analysis of the consolidated 3G score; black open triangles, HM-3F sequences determined by mixed population analysis of the consolidated 3F score; blue open circles, NH sequences determined by mixed population analyses of the consolidated 3G and consolidated 3F scores; †, general G→A hypermutation score (#G→A substitutions/#consensus G)/(#mutations/#nucleotides sequenced); *, 3G-specific G→A substitution score (#GG→AG substitutions/#consensus GG)/(#G→A/#G); ‡, 3F-specific G→A substitution score (#GA→AA substitutions/#consensus GA)/(#G→A/#G).