FIG. 5.

Viral dsRNA expression in cells stably expressing reovirus-specific siRNAs. Untransfected 293T (293T) cells and cell lines stably expressing S3 630, M1 895, M3 1861, or GFP siRNA (GFP) were infected with either T1L or T3D at an MOI of 10 PFU per cell and incubated for 18 h. The cytoplasmic fraction containing viral dsRNA from infected cells was resolved by electrophoresis in 10% polyacrylamide gels and analyzed by ethidium bromide staining. Size classes of viral dsRNA segments (large [L], medium [M], and small [S]) are indicated. Viral dsRNA extracted from purified T1L and T3D virions was used as a control. The results shown are representative of one experiment out of two performed.