FIG. 7.

Detection of protein expression in cells stably expressing M1 siRNA by immunofluorescence microscopy. 293T cells and cell lines stably expressing M1 895 siRNA were infected with either T1L or T3D at an MOI of 10 PFU per cell and incubated for 20 h. Infected cells were fixed and stained using anti-μ2 (rabbit) or anti-σNS (guinea pig) antiserum, followed by Alexa Fluor 488 goat anti-rabbit IgG (green) or Alexa Fluor 546 goat anti-guinea pig IgG (red). Cells were stained with TO-PRO3 (blue) to label nuclei.