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. 2006 Sep;80(18):9181–9191. doi: 10.1128/JVI.00295-06

FIG. 3.

FIG. 3.

Base insertions and second site alterations improve accumulation of CH5CCFV in protoplasts. (A) Base differences between satC H5 and CCFV H5 are boxed. Location of residues inserted at the base of CCFV H5 in CH5CCFV is indicated. (B) Effect of a second site mutation on accumulation of CH5CCFV. Protoplasts were inoculated with satCE (satC with 2 bases altered downstream of H5 required for inserting CCFV hairpins into satC), CH5CCFV, and CH5CCFVG218C, and levels of accumulating satRNA were determined at 40 hpi. (C) Accumulation of CH5CCFV with inserted cytidylates and adenylates flanking the base of H5 as shown in panel A. (D) Accumulation of CH5CCFV with single inserts upstream or downstream of H5. rRNAs were used as a loading control. Values below the lanes denote the average satC percentages of at least two replicates. None, no satRNA added to the inoculum.