FIG. 10.

AAV1 capsid glycan specificity on glycan array. A total of 264 glycans were screened for binding to the capsids as described in Materials and Methods. The plot shows the average relative fluorescence (RFU) for the six addresses for each glycan (thick line) versus glycan number. The standard error measurement (SEM) in the fluorescence for the six addresses (thin line) is drawn at the top of each thick line. The four top hits (printed array addresses: 1, AGP; 2, AGP-A; 6, transferrin; 215, NeuAcα2-3GalNAcβ1-4GlcNAcβ) with acceptable SEMs are indicated, with their relative fluorescence levels given in parentheses. All glycans recognized contain a common motif: sialic acid linked to N-acetyl-lactosamine.