FIG. 2.

Neuraminadase treatment of cells reduces AAV1 and AAV6 binding and transduction. (A, B, and C) Transduction assay. Cell surface sialic acid was removed from HepG2 (A), Pro-5 (B), and Cos-7 (C) cells by neuraminadase from Vibrio cholerae prior to transduction with AAV1-luc, AAV6-luc, and AAV2-luc. Twenty-four hours after transduction, cells were analyzed for luciferase expression with a luminometer. RLU, relative light units. (D) Cell binding assay. Pro-5 cells were treated with neuraminidase from Vibrio cholerae or mock treated prior to adding AAV1-luc, AAV6-luc, and AAV2-luc at 4°C. Virus binding was determined by quantitative dot blot hybridization. Values are means from three experiments. Error bars represent standard deviations.