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AAV transduction of resialylated sialic acid-deficient Lec-2 cells. The following sialyltransferases were used to add specific sialic acids to the surfaces of Lec-2 cells: α2,3(O)-sialyltransferase, α2,3(N)-sialyltransferase, and α2,6(N)-sialyltransferase. Resialylated Lec-2 cells were then transduced (MOI, 1,000) with either AAV1-luc or AAV6-luc (A), AAV4-luc (B), or AAV2-luc (C), and luciferase expression was measured 24 h posttransduction. RLU, relative light units.
