TABLE 4.

Comparison of cervid prion inocula in Tg mice expressing human, bovine, ovine, and murine PrP genes

Transgenic line	PrP expression (n-fold)	Inoculum	Incubation time ± SEM (days)	No. of animals ill/no. of animals inoculateda
Tg(HuPrP)440	2b	Elk4	>540	0/11 A
		Elk5	>540	0/13 B
		Elk6	>532	0/8
		Elk7	>538	0/7
		MD4	>512	0/9
		MD5	>512	0/6
		WTD4	>512	0/5
		WTD5	>539	0/8
		sCJD, M/M129	154 ± 2.6	18/18
Tg(BoPrP)4092	10c	Elk4	>622	0/6
		Elk5	>600	0/11 C
		Elk6	>610	0/3 D
		Elk7	>610	0/6
		MD4	>512	0/8
		MD5	>532	0/8 E
		WTD4	>532	0/7
		WTD5	>532	0/7
		BSE PG31/90	244 ± 4.8	9/9
Tg(BoPrP)4125	8 to 16d	Elk4	>610	0/10 F
		Elk5	>620	0/8
		BSE PG31/90	240 ± 5.3	41/41
Tg(OvPrP,VRQ)338	12e	Elk4	>516	0/5 G
		Elk5	>509	0/8 H
		Elk6	>509	0/11
		MD4	>516	0/9
		MD5	>516	0/11
		WTD4	>505	0/8
		WTD5	>505	0/11 I
		WTD6	>526	0/12
		Scrapie 027	178 ± 9.3	16/16
Tg(MoPrP)4053	8f	Elk4	542 ± 32.1	6/8
		Elk5	>590	0/10
		Elk6	>512	0/7 J
		Elk7	>512	0/8
		MD4	>539	0/5
		MD5	>512	0/5
		WTD4	>512	0/3 K
		WTD5	>532	0/10
		RML	59 ± 1.5	10/10

^aAnimals with intercurrent illness at later stages of the experiments died on the indicated days postinoculation (unless otherwise noted, one animal died on the indicated day): A, 428 days; B, 481 days; C, 589 days; D, 588 days (two animals); E, 399 days; F, 384 days; G, 446 and 494 days; H, 232 days (two animals); I, 291 days; J, 518 days; K, 224 days. These animals were determined to be free of prion disease after neuropathologic analysis for spongiform degeneration and immunohistochemistry for PrP and GFAP.

^bCompared to PrP^C in human brain (47).

^cCompared to PrP^C in wild-type FVB mouse brain.

^dCompared to PrP^C in bovine brain (43).

^eCompared to PrP^C in sheep brain, listed as 6× for the hemizygous line Tg(OvPrP,VRQ^+/0)338 (26).

^fCompared to PrP^C in wild-type FVB mouse brain (46).