TABLE 2.
Expression analysis of MMTV common integration site genes in wild-type- and ITAM mutant-induced tumorsa
| Virus | wnt1 total | wnt1 alone | fgf3 total | fgf3 alone | wnt1+fgf3+ | wnt1 and fgf3 negative | Time to tumor (days) |
|---|---|---|---|---|---|---|---|
| HPb | |||||||
| 1 | + | − | + | − | + | − | 188 |
| 2 | + | − | + | − | + | − | 188 |
| 3 | + | − | + | − | + | − | 194 |
| 4 | + | − | + | − | + | − | 195 |
| 5 | + | − | + | − | + | − | 240 |
| 6 | − | − | + | + | − | − | 251 |
| 7 | − | − | + | + | − | − | 251 |
| 8 | − | − | + | + | − | − | 265 |
| 9 | + | − | + | − | + | − | 270 |
| 10 | − | − | + | + | − | − | 270 |
| 11 | + | − | + | − | + | − | 284 |
| 12 | + | + | − | − | − | − | 313 |
| 13 | − | − | + | + | − | − | 317 |
| 14 | − | − | − | − | − | + | 337 |
| 15 | + | − | + | − | + | − | 351 |
| 16 | − | − | − | − | − | + | 405 |
| 17 | − | − | + | + | − | − | 455 |
| 18 | + | + | − | − | − | − | UN |
| Y1Y2c | |||||||
| 1 | − | − | + | + | − | − | 304 |
| 2 | + | − | + | − | + | − | 313 |
| 3 | − | − | + | + | − | − | 317 |
| 4 | − | − | − | − | − | + | 390 |
| 5 | + | + | − | − | − | − | 390 |
| 6 | − | − | − | − | − | + | 405 |
| 7 | − | − | + | + | − | − | 405 |
| 8 | − | − | − | − | − | + | 412 |
| 9 | + | + | − | − | − | − | 422 |
| 10 | − | − | + | + | − | − | 424 |
| 11 | − | − | + | + | − | − | 443 |
| 12 | − | − | − | − | − | + | 470 |
| 13 | + | + | − | − | − | − | 470 |
| 14 | − | − | − | − | − | + | 525 |
Tumors were analyzed for expression of the indicated oncogenes by RT-PCR as described in Materials and Methods. UN, unknown.
The expression turn-on percentages for HP viruses were the following: wnt1 total, 55.6; wnt1 alone, 11.1; fgf3 total, 77.8; fgf3 alone, 44.4; wnt1 and fgf3 positive, 33.3; wnt1 and fgf3 negative, 11.1. The expression turn-on percentages for Y1Y2 viruses were the following: wnt1 total, 28.6; wnt1 alone, 21.4; fgf3 total, 42.9; fgf3 alone, 35.7; wnt1 and fgf3 positive, 6.7; wnt1 and fgf3 negative, 35.7.
P values for HP versus Y1Y2 viruses were the following: wnt1 total, 0.127 (Pearson's chi-square statistic); wnt1 alone, 0.631 (two-sided Fisher's exact test); fgf3 total, 0.043 (Pearson's chi-square statistic); fgf3 alone, 1.000 (two-sided Fisher's exact test); wnt1 and fgf3 positive, 0.044 (Pearson's chi-square statistic); wnt1 and fgf3 negative, 0.195 (two-sided Fisher's exact test).