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Representative assortment of transposon insertion mutant phage genomes analyzed by HpaI digestion and agarose gel electrophoresis. Integration of a single LacZ′-Mu(NotI) transposon into a given restriction fragment adds 460 bp to the size of the fragment. Wild-type HpaI restriction fragment sizes are (fragment 1) 3,053 bp, (fragment 2) 2,393 bp, (fragment 3) 2,361 bp, (fragment 4) 1,451 bp, and (fragment 5) 819 bp.
