FIG. 2.

Fine mapping of transposon integration sites within the PM2 genome. The nucleotides of the circular genome are numbered starting from the unique EcoRII restriction site (1) and proceeding clockwise (38). Genes encoding structural and nonstructural proteins (Roman numerals) and ORFs (lowercase letters) are visualized as black, gray, and white arrows, respectively, indicating the direction of transcription. The origin of replication (ORI) and the known promoters (bent arrows) are indicated. The inset depicts an enlarged view of ORFs k and l. The transposon mutants are indicated by their clone numbers. The exact locations are shown in Table 1. Color-coded mutants represent the phenotypical classes described in the legend to Fig. 4.