FIG. 5.

Effects of IFN and cMed on protein synthesis. (a) Vero, MDBK, and MDBK/V cells were untreated (mock, from lane 1 to lane 4) or treated for 16 h with uIFN-α (from lane 5 to lane 8) or cMed (from lane 9 to lane 12) and then infected with HSV-1 [17] at an MOI of 10. At the time points indicated (hpi), cells were labeled and lysed and lysates were run on SDS-PAGE gels for analysis of total protein synthesis. As a control, cells were pretreated as described but left uninfected (lanes 1, 5, and 9, respectively). (b) Cell lysates corresponding to MDBK cells, mock or uIFN pretreated, from the previous experiment were separated by SDS-PAGE and probed by Western blotting for ICP0. Un, uninfected. (c) MDBK cells mock or uIFN-α pretreated for 16 h were infected as described above, and at the time points indicated, cytoplasmic RNA was extracted. RT-PCR was used to specifically amplify for ICP0 mRNA.