FIG. 4.

GST-vhs fusion protein cleaves RNAs 7 and 8 similarly to RNase A. (A and B) Left, secondary structure of either RNA 7 (A) or RNA 8 (B) oligonucleotides as predicted by the mfold program; the circle marks the sites of RNase A predicted cleavage. Right, 0.1 μg/μl of GST-vhs fusion protein (lanes 2 and 3), GST alone (lanes 4 and 5), or GST-mvhs mutant protein (lanes 6 and 7) as well as 0.01 μg/μl RNase A (lanes 8 and 9) were incubated with either 5′-end-labeled RNA 7 substrate (A) or 5′-end-labeled RNA 8 substrate (B) for the time intervals shown (in minutes). Lane 1, 5′-end-labeled Decade RNA marker. The lengths of the fragments (in nucleotides) are reported on the side.