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Time course of activation of EBV lytic replication by wild-type ZEBRA (WTgZ) and the Z(K188A) mutant. (A) Cell extracts prepared 48, 72, and 96 h after transfection with 6 μg of plasmid DNA were probed for Rta, EA-D, and FR3 expressed from the virus (v) and ZEBRA from the transfected plasmids [Z (p)] by immunoblotting. (B) A Southern blot of a BamHI digest of cellular DNA harvested 48, 72, and 96 h after transfection was probed with a subfragment of Xho 1.9 or EBV BamHI W (C).
