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. 2006 Sep;80(18):9300–9309. doi: 10.1128/JVI.02449-05

FIG. 1.

FIG. 1.

Expression of M-T2 in stably transfected Jurkat T-cell lines and susceptibility to apoptosis. (A) M-T2 expression in stably transfected Jurkat T-cell clones T2O-a, T2O-11, T2L-4, T2L-3, and Jneo analyzed by Western immunoblotting and densitometry. Percent apoptosis induced in M-T2-expressing and control Jurkat T cells after exposure to 10,000 J of UV irradiation, 20 μM etoposide (VP-16), and 10 μM CHX for 8 h. Data shown are means ± standard deviations and are representative of replicate experiments. (B) Susceptibility of Jneo (unfilled squares) and M-T2-expressing Jurkat T cells (filled symbols) after 8 h of culture in LZ-TRAIL, LZ-FasL, LZ-CD40L (control), or recombinant human TNF-α. Data are means ± standard errors of the means, which were ≤5%, and error bars have been omitted for clarity. (C) Flow cytometry analysis of death receptor expression in Jurkat T-cell clones. Histograms show death receptor expression on the control Jneo Jurkat line (solid lines), both at the cell surface and intracellularly, relative to staining with isotype control antibodies (filled histograms). Death receptors on M-T2-expressing Jurkat lines T2O-a (dots), T2O-11 (spaced dots), T2L-4 (small dashes), and T2L-3 (large dashes) are overlaid with control Jneo cells (solid lines).