. 2005 Oct 4;37(1):73–83. doi: 10.1016/j.ymeth.2005.05.008

Table 5.

Reagents used in cellular lysis

Blood, plasma sample, and saliva	Directly add alkaline lysis solution at 1:9 ratio to break the cell membrane. Blood lysates are very viscous so the probe solution has to be added immediately after lysis	0.4 M NaOH (Sigma S0899)
Sputum	Add PBS buffer at 1:1 ratio then alkaline lysis solution at 1:9 ratio to buffered sputum	0.4 M NaOH (Sigma S0899)
Urine and cell culture	Universal lysis buffer containing 1% Triton X-100, 0.1 M KH₂PO₄, 2 mM EDTA, and 1 mg/mL lysozyme	Triton X-100 (Sigma T9284), KH₂PO₄ (Sigma P0662), EDTA (Sigma E-5134), lysozyme (Sigma, L-6876)