Fig. 4.

Comparison between calculated and measured Δg_tr values for osmolytes. (A) Δg_tr values, calculated from the model, are plotted against experimentally determined values from Table 1. Good agreement is apparent. The linear regression line (solid line) is given by Δg_tr^measured = 0.81 Δg_tr^calculated − 3.2, with correlation coefficient 0.99. Data points corresponding to the 10 osmolytes in Fig. 1 are annotated in Inset. (B) Calculated Δg_tr values on higher osmolyte concentrations (>1 M) are plotted against available experimental data for sarcosine (indigo triangles), urea (green squares), and guanidine (orange asterisks). Solid lines were drawn from model-based Δg_tr values, extended beyond 1 M osmolyte concentrations.