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Prevention of cell death by IGF-II overexpression. Confluent C2C12-1.1 and -2.7 cells were treated with or without cisplatin (25 µM) in 10% FBS medium at indicated time periods. Attached and floating cells were collected and analyzed on a FACScan flow cytometer for relative DNA content. The means±SE of triplicate determinations are shown. *P < 0.025; **P <0.01; ***P <0.001.
