Figure 3.
Rapamycin abrogates the IGF-II-mediated protection of cells to cisplatin-induced apoptosis. (A) C2C12-1.1 and -2.7 cells were preincubated with rapamycin (100 nM) for 3 hours and then treated with or without cisplatin (25 µM) for 24 hours in 10% FBS medium. Cells were stained with propidium iodide and then analyzed by flow cytometry as described in Materials and Methods. The area labeled M1 is an apoptotic population, M2 is G1, M3 is S, and M4 is G2/M phase population. (B) The means±SE of triplicate determinations are shown. *P <0.005; **P>0.05. (C) Cells were preincubated with rapamycin (100 nM) for 3 hours and then treated with or without cisplatin (25 µM) for 24 hours in 10% FBS medium. Cell lysates were subjected to Western blotting with antibodies against phospho-p70 S6K and actin.