Expression of Sema7A transcripts in E3.5 chick embryos. A: In situ hybridizations were performed on whole mount E3.5 chick embryos. Section planes are shown with broken arrows and the results of section after in situ hybridization are shown in B–D (1–3). aer, apical ectodermal ridge; sec, surface ectoderm; dm, dermomyotome; nc, dorsal-laterally migrated neural crest cells; vnc, ventral-medially migrated neural crest cells. Scale bars = 100 μm.