Fig. 4.

T0901317 and ALLO promote Purkinje cell survival in npc1^−/− mice. Cerebellar sections from 49-day-old WT mice (Top), vehicle-treated npc1^−/− mice (Middle), and T0901317 and ALLO-treated npc1^−/− mice (Bottom) were stained with antibodies to CD68 (green) and calbindin (red) and with a nuclear stain, Hoechst dye 33342 (blue in Left), and examined by immunofluorescence. The asterisk and diamond denote granule and Purkinje cell layers, respectively. Arrowheads indicate calbindin-stained Purkinje cell bodies. Omission of the nuclear stain (Right) facilitates visualization of microglia within the granule cell layer. (Scale bar: 200 μm.)