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Pretreatment with heat-killed staphylococci protects animals from infection. Mice were pretreated with i.p. injection of either buffer (PBS) or heat-killed wild-type (WT) or lgt mutant bacteria 24 h before i.v. challenge with live (5 × 106) WT or lgt mutant bacteria. The percentage of survival after challenge was recorded over a 6-day time course.
