Fig. 1.

AnxA5 binding to L. major promastigotes. Populations of L. major promastigotes were stained with AnxA5-Fluos. Flow cytometry histogram profiles of stat. phase promastigotes (black line, a) and of stat. phase-derived metacyclic promastigotes (met, black line, b). The dotted lines (a and b) show the control staining in the absence of Ca²⁺. (c) Metacyclic promastigotes derived from P. duboscqi sandflies (black line) as described (22). The dotted line shows the unstained control. (d) Confocal micrograph (0.15-μM slice) of a promastigote-stained positive with AnxA5 (arrow) and an AnxA5− promastigote. (Scale bar, 5 μm.) (e) Flow cytometry densitoblot showing forward scatter (FSC-H) and sideward scatter (SSC-H) analysis of the stat. phase promastigotes. (f) AnxA5-Fluos binding of the population A in e (white histogram) and population B in e (filled histogram).