Fig. 3.

mZip14 siRNA decreases Zip14 expression in AML12 mouse hepatocytes. (A) QRT-PCR analysis of Zip14 mRNA levels in AML12 cells treated for 96 h with a nontargeting (control) siRNA or siRNA targeted to mZip14. Values are the mean ± SD of three separate experiments, each with n = 3. ∗, P < 0.05 compared with control. (B) Cells in parallel were analyzed for Zip14 protein levels by Western blotting of total cell lysates (50 μg of protein per lane). To confirm equivalent protein loading, the Western blot was stripped and reprobed with anti-tubulin antibody. A representative experiment is shown.