Table 3.

IFG-treated N370S GlcCerase has an altered response to SDS

Cell type	pH	IFG	Relative GlcCerase activity
WT	5.2	−	0.78 ± 0.04
	6.4	−	0.90 ± 0.04
N370S	5.2	−	0.338 ± 0.082∗
		+	0.275 ± 0.093∗
	6.4	−	2.96 ± 0.58†
		+	2.11 ± 0.62†

Cell lysates from wild-type (WT), N370S, and IFG-treated N370S fibroblasts were incubated with or without 1.0 mM SDS at room temperature for 30 min before measurement of GlcCerase activity. Results are presented as SDS/control. Separate cell lysates were prepared for experiments at either pH 5.2 or pH 6.4.

∗, P < 0.05 (n = 4);

†, P = 0.001 (n = 5).