Fig. 1.
Detection of ADP-ribosylated DNA adducts formed with crude extracts of clams. Calf thymus DNA was reacted with 32P-NAD in the presence of 1 μg of crude extract protein. The DNA samples were spotted on TLC sheets with and without nuclease digestion, which were developed and subjected to autoradiography. Sample 1, no protein; sample 2, pierisin-1 (10 pg, positive control); sample 3, M. lamarckii; sample 4, R. philippinarum; sample 5, C. japonica; sample 6, My. galloprovincialis. The arrow indicates the Rf value of 0.05. +, with nuclease digestion; −, without nuclease digestion.